Remya T., Kunhi, A.A.M. Asha, B., Shainy N.K., and Farha Barami (2017). Production, partial purification and partial characterization of a thermo stable and thermo active alkaline protease from Bacillus sp. S-CSR-0001 isolated from soil. In P.J. Jisha and K. Mashhoor (Eds.) ‘Advances in Microbiology’ LAP LAMBERT Academic Publishing, Saarbrucken, Germany, pp. 28-46 (ISBN: 978-3-330-07223-7).

 

 

Abstract

A bacillus species isolated from soil designated as Bacillus sp. S-CSR-0001was found to be producing thermo stable alkaline protease enzyme. The enzyme showed its maximum activity at a pH of 9.5, and temperature 60 oC.  Skim milk powder was found to be the appropriate substrate for maximum enzyme production at 2% concentration. The enzyme activity remained stable up to a temperature of 55 °C and a pH of 9.5. The enzyme was partially purified by ammonium sulphate precipitation (80%) method, followed by protease assay and estimation. The specific activity of partially purified protease was 1500 U/mg. The study confirmed that the isolated Bacillus sp. S-CSR-0001 could be a good source of thermo active alkaline protease which can be exploited for pharmaceutical and industrials applications.