Hasna T., Sajina A., Lintu Josy and Kunhi A.A.M. 2016. Callus Proliferation and Stimulation of Secondary Metabolites in Aquilaria agallocha Roxb. (Agarwood Tree). Abstract Book. National symposium on ‘Biosciences and Technology –Recent Developments and Future Prospects’, SIAS-Centre for Scientific Research, Safi Institute of Advanced Study, Vazhayoor, Malappuram, Kerala, p.25.

ABSTRACT

An efficient protocol for callus proliferation and stimulation of secondary metabolites in Aquilaria agallocha Roxb. has been standardized using leaf explants. Experiment was performed to examine the effect of growth regulators on callus induction, proliferation and stimulation of secondary metabolite s in a shorter time period. Callus proliferation (100%) within 95 days was observed on MS medium supplemented with BAP (1.2mg/l) and NAA (0.05mg/l). Secondary metabolite stimulation (70%) was achieved on MS medium supplemented with 2, 4-D (2.0 mg/l). 

 

Keywords: Callus proliferation, Secondary metabolite, Aquilaria Malaccencis, Agarwood tree.