Mashitha P.K., Asha B.A. and Kunhi A.A.M. 2016. Production and Partial Characterization of Alkaline Protease from Pseudomonas aeruginosa S-CSR-0009 Isolated from Soil. Abstract Book. National symposium on ‘Biosciences and Technology –Recent Developments and Future Prospects’, SIAS-Centre for Scientific Research, Safi Institute of Advanced Study, Vazhayoor, Malappuram, Kerala, p. 28.


A Pseudomonas species isolated from soil designated as Pseudomonas aeruginosa S-CSR-0009 was found to be producing alkaline protease enzyme. The enzyme showed its maximum activity at a pH of 9, and temperature 40o C. Skim milk powder was found to be the appropriate substrate for maximum enzyme production at 3% concentration. The enzyme activity remained stable up to a temperature of 40°C and a pH of 9. The study confirmed that the isolated Pseudomonas aeruginsa S-CSR-0009 could be a good source of alkaline protease which can be exploited for pharmaceutical and industrials applications. 


Keywords: Alkaline protease, Partial Characterization, Pseudomonas aeruginosa.