Rubeena K.T., Smitha K.V., Sooraj S. Nath and Kunhi A.A.M. 2016. Partial Characterization of Raw Starch Hydrolyzing Enzyme Produced by Bacillus S-CSR-0006. Abstract Book. National symposium on ‘Biosciences and Technology –Recent Developments and Future Prospects’, SIAS-Centre for Scientific Research, Safi Institute of Advanced Study, Vazhayoor, Malappuram, Kerala, p. 54.

ABSTRACT

This study reports the isolation and characterization of a raw starch digesting amylase from a newly isolated organism, screened for amylase production by observing the halo zone appeared around the colonies. From the morphology, staining and biochemical characteristics, the organism was identified as Bacillus sp. coded as Bacillus S-CSR-0006 gave the amylase yield (820U/ml) in submerged fermentation process, hence it was selected for further studies. Amylase from Bacillus sp.S-CSR-0006, isolated from soil contaminated with canteen kitchen waste showed an ability to degrade tapioca, corn, arrow root, rice starches. Tapioca has the highest degree of hydrolysis followed by corn, rice, arrow root, consecutively. The crude amylase preparation had temperature and pH optimal activities at 40°C and 9 respectively, stabilities at 35C and 8 respectively. The highest adsorption of crude enzyme was found with rice starch followed by arrow root, corn and tapioca. The optimum substrate concentration was 1%. Crude enzyme was partially purified by 20-100% ammonium sulphate precipitation followed by dialysis, in which amylolytic activity shown by 100% was 875 U/ml. 

 

Keywords: Bacillus S-CSR-0006, Amylase.